Experience
Postdoctoral Research with Dr. Daniel Appella
National Institutes of Health, NIDDK, Bethesda MD
Project Title: Developing cyclopentane-modified peptide nucleic acids as diagnostic tools
Associate Scientist
Tasso Inc, Seattle WA
Project Title: Patient-Centered Leukemia Diagnostic And Monitoring Using The Hemolink-MD
Graduate Research with Dr. Bruce Armitage
Carnegie Mellon University, Pittsburgh PA
Project Title: The Design, Synthesis, and Characterization of Bright Fluorescent Probes for Biomolecule Detection
Undergraduate Research with Dr. Massimiliano Lamberto
Monmouth University, West Long Branch, NJ
Project Title: Synthesis of Small Organic Molecules as Potential Stabilizers of DNA G-Quadruplex
Undergraduate Research with Dr. M. Lamberto and Dr. F.M. Raymo
University of Miami, Coral Gables, FL
Project Title: Synthesis of Photocaged Chromophores
Publications
Zheng, H.; Botos, I.; Clausse, V.; Nikolayevskiy, H.; Rastede, E.; Fouz, M.; Appella, D. “Conformational Constraints of Cyclopentane Peptide Nucleic Acids Facilitate Tunable Binding to DNA” Nucleic Acid Research 2020.
Shank, N.I.; George-Rosenker, K.M.; Englund, E.A.; Dix, A.V.; Rastede, E.E.; Appella, D.H.; “Synthesis and Application of LKγT Peptide Nucleic Acids”. Book Chapter. Non-Natural Nucleic Acids: Methods and Protocols. 2019.
Orenstein, A.; Berlyoung, A. S.; Rastede, E. E.; Pham, H. H.; Fouquerel, E.; Murphy, C.T.; Leibowitz, B.J; Yu, J.; Srivastava, T.; Armitage, B. A.; Opesko, P.L.; “γPNA FRET Pair miniprobes for Quantitative Fluorescent in Situ Hybridization to Telomeric DNA in Cells and Tissue”. Molecules (2017), 22, 2117-2132.
Gupta, P.; Rastede, E. E.; Appella, D. H.; “Multivalent LKγ-PNA oligomers bind to a human telomere DNA G-rich sequence to form quadruplexes.” Bioorg. Med. Chem. Lett. (2015), 25, 4757-4760.
Rastede, E. E.; Tanha, M.; Yaron, D.; Watkins, S. C.; Waggoner, A. S.; Armitage, B. A., Spectral fine tuning of cyanine dyes: electron donor-acceptor substituted analogues of thiazole orange. Photochem. Photobiol. Sci. (2015), 14, 1703-1712.
Lamberto M; Rastede, E.E.; Decker, J. and Raymo, F.M. “Microwave assisted synthesis of symmetric and asymmetric viologens”, Tetrahedron Letters, (2010), 51(42), 5618-5620.
Conference Publications
Undergraduate co-authors are underlined.
257th ACS National Meeting, Orlando, FL (2019) Rastede, Elizabeth E; Lampon, Marcella; Bethier, Erwin; Appella, Daniel. “Design, synthesis, and evaluation peptide-based Fluorescence Resonance Energy Transfer (FRET) probes for the development of protease activity assays”.
254th ACS National Meeting, Washington, DC (2017) Gould, Michael; Rastede, Elizabeth E; Appella, Daniel; “Development Of Peptide Nucleic Acids For The Optimization Of Assay Sensitivity For Quantitative Determination Of HIV Viral Load”.
NIH Summer Research Symposium (2016) Pasternak, Evan; Rastede, Elizabeth; Appella, Daniel; “Optimization of a Nucleic Acid Test for the Detection of HIV using a PNA based Enzyme-Linked Assay”
Meeting of the Minds CMU Symposium (2015) Moon, Michelle; Rastede, Elizabeth; Armitage, Bruce; “Characterization Of Internally-Labeled Peptide Nucleic Acid (PNA) Fluorescent Probes”
ACS Central Regional Meeting, Pittsburgh, PA (2014). (Invited Speaker) Rastede, Elizabeth E; Armitage, Bruce A; Building bright fluorescent dye labeled peptide nucleic acid probes
248th ACS National Meeting, San Francisco, CA (2014) Rastede, Elizabeth E; Armitage, Bruce A; “PNA as a Versatile Tool for High Density Fluorescent Labeling” .
Fluorescent Biomolecules and Building Blocks Conference (2014). Rastede, Elizabeth E; Armitage, Bruce A; “PNA as a Versatile Tool for High Density Fluorescent Labeling ”.
Gordon Research Conference- Bioorganic Chemistry (2014). Rastede, Elizabeth E; Armitage, Bruce. A; “’Click PNA’: A Versatile Tool for High Density Fluorescent Labeling of Peptide Nucleic Acids
Meeting of the Minds CMU Symposium (2014) Ronner, Peter; Rastede, Elizabeth; Armitage, Bruce. “The Synthesis And Characterization Of Self-Assembling Bis-Intercalating Thiazole Orange Dimers”
Meeting of the Minds CMU Symposium (2014) Hong, Chris; U; Rastede, Elizabeth; Armitage, Bruce. “Optimization Of PNA Probes For Fluorescent Imaging”
National Technology Centers for Networks and Pathways -NIH investigators meeting (2013). Rastede, Elizabeth E.; Armitage, Bruce A. “Spectral Fine tuning of a Cyanine Dyes: Electron Donor-Acceptor Substituted Analogues of Thiazole Orange”.
National Technology Centers for Networks and Pathways Meeting (2012). Rastede, Elizabeth E.; Armitage, Bruce A.; “Fine tuning of a thiazole orange/ScFv fluoromodules.
63rd Southeast Regional Meeting of the American Chemical Society (2011). Rastede, Elizabeth E.; Lamberto, Massimiliano; Armitage, Bruce A. “Synthesis and binding of a quadruplex targeting naphthalene diimide ligand”
238th ACS National Meeting, Washington, DC (2009). Lamberto Massimilano; Rastede,Elizabeth E; Alite, Hekla; Karney Michael. “Microwave assisted synthesis of cationic naphthalene diimide”